Gavrilov Svetlana author Columbia University. Genetics and Development Marolt Darja author Columbia University. Biomedical Engineering Douglas Nataki C. author Columbia University. Obstetrics and Gynecology Prosser Robert W. author Columbia University. Obstetrics and Gynecology Khalid Imran author Columbia University. Medicine Sauer Mark V. author Columbia University. Obstetrics and Gynecology Landry Donald W. author Columbia University. Medicine Vunjak-Novakovic Gordana author Columbia University. Biomedical Engineering Columbia University. Medicine Papaioannou Virginia E. author Columbia University. Biomedical Engineering Columbia University. Obstetrics and Gynecology originator text Articles 2011 English We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion of in vitro fertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos. Obstetrics and gynecology Developmental biology 2011 765378 1 9 2011 http://dx.doi.org/10.4061/2011/765378 http://hdl.handle.net/10022/AC:P:14446 NNC NNC 2012-08-20 12:30:00 -0400 2012-08-20 12:50:25 -0400 8466 eng