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Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses

Thomas Briese; Andrew Rambaut; W. Ian Lipkin

Title:
Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses
Author(s):
Briese, Thomas
Rambaut, Andrew
Lipkin, W. Ian
Date:
Type:
Articles
Department:
Center for Infection and Immunity
Volume:
85
Permanent URL:
Book/Journal Title:
Journal of general virology
Abstract:
Guaroa virus (GROV), a segmented virus in the genus Orthobunyavirus, has been linked to the Bunyamwera serogroup (BUN) through cross-reactivity in complement fixation assays of S segment-encoded nucleocapsid protein determinants, and also to the California serogroup (CAL) through cross-reactivity in neutralization assays of M segment-encoded glycoprotein determinants. Phylogenetic analysis of the S-segment sequence supported a closer relationship to the BUN serogroup for this segment and it was hypothesized that the serological reaction may indicate genome-segment reassortment. Here, cloning and sequencing of the GROV M segment are reported. Sequence analysis indicates an organization similar to that of other orthobunyaviruses, with genes in the order GN–nsm–gC, and mature proteins generated by protease cleavage at one, and by signalase at possibly three, sites. A potential role of motifs that are more similar to CAL than to BUN virus sequences with respect to the serological reaction is discussed. No discernable evidence for reassortment was identified.
Subject(s):
Epidemiology
Virology
Publisher DOI:
http://dx.doi.org/10.1099/vir.0.80122-0
Item views:
126
Metadata:
text | xml

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